Scanning Electron Microscopy : A New Staple for Cell Biology?
Claire BOULOGNEInstitut de Biologie Intégrative de la Cellule (I2BC) - Université Paris-Saclay
Since the early beginnings of the technique, electron
microscopy has been a very important tool for cell biology, especially
to explore inside the cells.
Due to the restriction of sample preparation and resolution,
transmission electron microscopy (TEM) was for a long time the only
approach to analyse the interior of the cells at the nanometric scale.
On the other side, scanning electron microscopy was restricted to
surface analysis and was so only marginally used in cell biology.
With the latest development in SEM technology, this paradigm changed
and SEM is more and more used to study cell compartmentation. Indeed,
SEM offers many advantages for advanced techniques, like 3D analysis
and correlative microscopy.
In this talk, examples of application with a FEG will be presented.
programme juillet 2023