3D Ultrastructural Imaging dedicated to host pathogen studies
P. BOMME*, M. MAJROUH, E. PENARD, D TAHIR, V CHOUMET, Jost ENINGA, G DUMENIL, A.MALLET
Institut Pasteur, 28 Rue du Dr Roux, 75015 Paris
Microscopy and imaging in general are crucial
points for scientist to understand a specific biological event as entry
of a pathogen into cells or tissue and how vectors transmit diseases.
Electron microscopy (EM) has always been a method of choice to reach ultrastructural details 
More recently 3D EM technics can enhance and sometimes revolutionize how we view cells and biological processes .
In the last years, 3D imaging modalities became one of the main challenges for electron imaging community.
In our facility UBI, we proposed a large choice of 3D methods allowing
to visualize proteins, virus, cells or tissues. We’ll discuss about 3D
methods applied to biological samples and how 3D reconstruction allows
better understanding of pathogen associated processes.
 Santos JC et al. 2015
 Mallet et al. 2022
programme juillet 2023